http://en.wikipedia.org/wiki/Henderson–Hasselbalch_equation
or any intro chemistry text William G. Scott Professor Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA 228 Sinsheimer Laboratories University of California at Santa Cruz Santa Cruz, California 95064 USA On Feb 3, 2012, at 6:42 PM, megha goyal wrote: > Our recombinant product is formulated in 10 mM sodium acetate buffer at pH > 4.0 and the std composition mentions sodium 0.035 mg and acetate 0.59 mg per > ml of sample. It mentions Sodium acetate is formed by titrating glacial > acetic acid with sodium hydroxide. > > Can anyone guide me on how this 10 mM buffer is prepared or on its > calculation. > > What currently I do is the old method that we have been following i.,e > Use 0.123 mg Na Acetate trihydrate + 0.476 µl Gl. Acetic acid, adjust pH to > 4.0 using 5 N NaOH. But we do not know how these values have been arrived at > and if this calculation is correct. > > Please guide me with this regards. Any help on this will be highly > appreciated. > > > > thanks, > > > > > Megha >
