I assumed that since this topic came up fairly recently, in fact 3 weeks ago (see thread starting from http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg23628.html), it wasn't just a re-run of the same question.
Perhaps the original poster could clarify whether we are talking about unexplained -ve density in the 2Fo-Fc map or in the Fo-Fc map? Cheers -- Ian On 23 November 2011 16:03, Nat Echols <nathaniel.ech...@gmail.com> wrote: > On Wed, Nov 23, 2011 at 7:57 AM, Ian Tickle <ianj...@gmail.com> wrote: >> On 23 November 2011 07:54, Careina Edgooms <careinaedgo...@yahoo.com> wrote: >>> I have a question about a 2F0-Fc difference map that I calculated with >>> Refmac. >> >> On 23 November 2011 15:40, Nat Echols <nathaniel.ech...@gmail.com> wrote: >>> The negative density around Met S could be radiation damage. >> >> But you wouldn't expect to see -ve density in the 2Fo-Fc map from >> radiation damage right? The Fo-Fc map for sure. > > I assumed that's what the original poster meant. > > (and I apologize for the redundant comments, GMail groups messages by > subject, so every time someone changes the subject line, it becomes a > new thread, which I usually miss. That said, I thought after reading > Garib's Refmac paper published earlier this year that it was now using > distance-based B-factor restraints, instead of bond connectivity - is > this correct or did I misunderstand?) > > -Nat >
