On Wed, Nov 23, 2011 at 7:57 AM, Ian Tickle <ianj...@gmail.com> wrote: > On 23 November 2011 07:54, Careina Edgooms <careinaedgo...@yahoo.com> wrote: >> I have a question about a 2F0-Fc difference map that I calculated with >> Refmac. > > On 23 November 2011 15:40, Nat Echols <nathaniel.ech...@gmail.com> wrote: >> The negative density around Met S could be radiation damage. > > But you wouldn't expect to see -ve density in the 2Fo-Fc map from > radiation damage right? The Fo-Fc map for sure.
I assumed that's what the original poster meant. (and I apologize for the redundant comments, GMail groups messages by subject, so every time someone changes the subject line, it becomes a new thread, which I usually miss. That said, I thought after reading Garib's Refmac paper published earlier this year that it was now using distance-based B-factor restraints, instead of bond connectivity - is this correct or did I misunderstand?) -Nat
