I have the same problem as I am not using a robot. For this reason I use the 48 well hanging drops from Hampton and seal them one at a time. It is very time consuming but uses little reservoir solution and your drops do not dry out..
Jackie Vitali On Fri, Nov 18, 2011 at 7:24 PM, Ho Leung Ng <h...@hawaii.edu> wrote: > Hello Ivan, > > You can dispense 0.1-0.2 uL drops with the PB-100 repeating > dispenser from Hamilton and a 10 uL glass syringe. But it's rather > clumsy and hard on the hands. I don't recommend using it with 96 well > plates. It's too slow to do the whole plate at one time without your > drops drying up. > > > Ho > > Ho Leung Ng > University of Hawaii at Manoa > Assistant Professor, Department of Chemistry > holeung...@hawaii.edu > > > ------------------------------------------------------------------------------------ > From: xaravich ivan <xaravich.i...@gmail.com> > Subject: Manually setting 96 wells plates with lower volume samples! > > Hi, > I apologize in advance as it is not a ccp4 related question, but over the > years, CCP4bb is synonymous with protein crystallographers virtual > university, at least for me. > > Ok, now I do not have an easy access to crystallization robot, so I was > hoping if someone here have ever used the 96 well plates for manually > setting drops with much lower solution/sample volumes (0.1-0.2micro > litres). > > I heard about a clicker syringe that can be used to manually add lesser > volumes but I am not sure how to go about it. > Please let me know if you are aware of or are routinely setting 96 well > trays with much lower volumes of crystallization solutions as well as > samples, manually. > > thanks in advance, > ivan >
