Hi, With the electronic Biohit 8 channel pipette you can set up 3x96 drops in 5 minutes. Lowest volume is 0.2 ul. We have used this system for a couple of years with RNase free filter tips for RNA-protein complexes. With the recent arrival of a shared Mosquito we have used the Biohit less but it is still convenient to have the setup. You need good eyesight and a steady hand, though.
Cheers, Martin On Nov 18, 2011, at 3:35 PM, "xaravich ivan" <xaravich.i...@gmail.com> wrote: > Hi, > I apologize in advance as it is not a ccp4 related question, but over the > years, CCP4bb is synonymous with protein crystallographers virtual > university, at least for me. > > Ok, now I do not have an easy access to crystallization robot, so I was > hoping if someone here have ever used the 96 well plates for manually setting > drops with much lower solution/sample volumes (0.1-0.2micro litres). > > I heard about a clicker syringe that can be used to manually add lesser > volumes but I am not sure how to go about it. > Please let me know if you are aware of or are routinely setting 96 well > trays with much lower volumes of crystallization solutions as well as > samples, manually. > > thanks in advance, > ivan