AUC !
Andreas
On 05/09/2011 6:00, Jacob Keller wrote:
mea culpa! How about FRET?
JPK
On Mon, Sep 5, 2011 at 11:39 AM, Bosch, Juergen<jubo...@jhsph.edu> wrote:
Hi Jacob,
you forgot cross-linking to stabilize a weak complex and verify that it
exists.
Jürgen
On Sep 5, 2011, at 11:45 AM, Jacob Keller wrote:
Well, I guess I have always been curious what is the gold standard
here--perhaps SEC, ITC, SPR, pulldowns? What if SEC shows a
polydisperse sample with weak oligomerization, or SPR a very weak
binding constant? Do we then revert to a functional assay? Or what if
the functional assay does not show anything, but the binding constant
is really strong? Or vice versa, the binding is completely
undetectable, but the functional assay shows something?
JPK
--
Andreas Förster, Research Associate
Paul Freemont & Xiaodong Zhang Labs
Department of Biochemistry, Imperial College London
http://www.msf.bio.ic.ac.uk
