I was playing around with PDBe PISA and came across the following:
For pdb entry 1OYA. The most promising interface has an area bury of around
720A^2 and DeltaG of -10.6Kcal/mol. sym_op(y,x,-z+1) and CSS of 0.039!
Assembly analysis says it has no strong indications that point to stable
quaternary structure.
This protein has been extensively studied and determined to be a dimer.
Entry 3RND is the same protein with one single conservative mutation deep in
the active site.
They align with a RMSD of 0.3 A, 99.8% sequence identity. Superposition and
inspection of the regions that contact
the adjacent monomer shows they are basically identical.
The interface here shows Area bury of 760 A^2 and DeltaG = -6.6Kcal/mol. sym_op
(-y,-x,-z-1/2) CSS=0.00 !
Assembly analysis basically says no stable oligomers form. This enzyme also is
dimer according to gel filtration.
Could anyone ellaborate on this please, if they feel like they have the time...
Cheers
