Dear all, I have facing one problem during the refinement of my protein . Actually in my protein there are some modified amino acids are present like Cystein is modified into CME which i can get easily from monomer libraray in coot . but after refinement in Pdb text file indicated some gaps while in the structures there are no gap in between these amino acids so if any one suggest me what to do. I would appreciate your kind suggestions.
LINKR GLU A 142 LEU A 144 gap LINKR SER A 328 GLY A 330 gap LINKR LEU A 138 GLU A 140 gap LINKR GLU A 126 ASP A 130 gap LINKR SER A 246 GLY A 248 gap Many thanks for your time Best regards Afshan