You do need to try space group I21 21 21 as well as these two space groups are indistinguishable from systematic absences Phil
On 24 Jun 2011, at 08:50, mullapudi edukondalu wrote: > Dear Members, > > I have my first data set on one of my protein crystals, that diffract to > 2.7 A, and the space group is I222. According to Mathews coefficient, there > should be 4 molecules in the asymmetric unit. But, when I run molecular > replacement programme it found only 3, and the crystal packing is not that > good. I have 100% similar model. And Ref mac gave me an Rfree of 46%. Could > any one of you please suggest me how to go further with this problem? Looking > forward to hear from you. > > Best Regards > > Edukondalu Mullapudi > > -- > Edukondalu Mullapudi > PhD Student > Molecular Host-Pathogen Interactions (MHPI) > Division of Structural Biology (SB) > Helmholtz-Centre for Infection Research (HZI) > Inhoffenstr. 7 > 38124 Braunschweig > Germany > Phone:+4953161817041 > Mobile:+4917622997722
