Dear Members, I have my first data set on one of my protein crystals, that diffract to 2.7 A, and the space group is I222. According to Mathews coefficient, there should be 4 molecules in the asymmetric unit. But, when I run molecular replacement programme it found only 3, and the crystal packing is not that good. I have 100% similar model. And Ref mac gave me an Rfree of 46%. Could any one of you please suggest me how to go further with this problem? Looking forward to hear from you.
Best Regards Edukondalu Mullapudi -- Edukondalu Mullapudi PhD Student Molecular Host-Pathogen Interactions (MHPI) Division of Structural Biology (SB) Helmholtz-Centre for Infection Research (HZI) Inhoffenstr. 7 38124 Braunschweig Germany Phone:+4953161817041 Mobile:+4917622997722
