Dear Crystallogrpahers, thanks for all of the responses to this perennial crystallographic question (is my crystallogrpahic assembly biologically-relevent). Summarized, they were:
Joel Janin's work, particularly: --Acta Cryst 2007, --The atomic structure of protein-protein recognition sites. Lo Conte L, Chothia C, Janin J. J Mol Biol. 1999 Feb 5;285(5):2177-98. --Protein-protein interaction and quaternary structure. Janin J, Bahadur RP, Chakrabarti P. Q Rev Biophys. 2008 May;41(2):133-80. Review. --Proteins. 1995 Dec;23(4):580-7. Protein-protein interaction at crystal contacts. Janin J, Rodier F. --J Mol Biol. 2004 Feb 27;336(4):943-55. A dissection of specific and non-specific protein-protein interfaces. Bahadur RP, Chakrabarti P, Rodier F, Janin J. --Proteins. 2005 Jul 1;60(1):36-45. Hydration of protein-protein interfaces. Rodier F, Bahadur RP, Chakrabarti P, Janin J. And also a paper from Eugene Krissinel: --J.Comp.Chem. 31(1),133-143 On Thu, May 26, 2011 at 7:50 AM, Kushol Gupta <kushol.gu...@gmail.com> wrote: > Hi Jacob - > > These references might(?) be helpful: > > Proteins. 1995 Dec;23(4):580-7. Protein-protein interaction at crystal > contacts. Janin J, Rodier F. > J Mol Biol. 2004 Feb 27;336(4):943-55. A dissection of specific and > non-specific protein-protein interfaces. Bahadur RP, Chakrabarti P, Rodier > F, Janin J. > Proteins. 2005 Jul 1;60(1):36-45. Hydration of protein-protein interfaces. > Rodier F, Bahadur RP, Chakrabarti P, Janin J. > > The problem you describe is a tricky one - crystal packing can be deceiving. > Complementing your structural studies with some biophysical measurements > (ie: comparing computed hydrodynamic properties to those observed in gel > filtration or centrifugation or SAXS or the like) could help flesh out these > types of questions. > > Hope this helps, > > Kushol > > Kushol Gupta, Ph.D. > Research Associate > Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine > kgu...@mail.med.upenn.edu > 215-573-7260 / 267-259-0082 > > > -----Original Message----- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob > Keller > Sent: Wednesday, May 25, 2011 10:42 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] Histogram/Plot of Buried Surface Areas > > Dear Crystallographers, > > is anyone aware of a reference or plot addressing buried surface area > (or PISA output values) versus veracity of a complex? I am trying to > determine the physiological relevance of a > crystallographically-observed assembly, and would love to put my PISA > output in the context of verified complexes versus crystal contacts. > > Thanks, > > Jacob > > ******************************************* > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > cel: 773.608.9185 > email: j-kell...@northwestern.edu > ******************************************* > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
