Hi Jacob - These references might(?) be helpful:
Proteins. 1995 Dec;23(4):580-7. Protein-protein interaction at crystal contacts. Janin J, Rodier F. J Mol Biol. 2004 Feb 27;336(4):943-55. A dissection of specific and non-specific protein-protein interfaces. Bahadur RP, Chakrabarti P, Rodier F, Janin J. Proteins. 2005 Jul 1;60(1):36-45. Hydration of protein-protein interfaces. Rodier F, Bahadur RP, Chakrabarti P, Janin J. The problem you describe is a tricky one - crystal packing can be deceiving. Complementing your structural studies with some biophysical measurements (ie: comparing computed hydrodynamic properties to those observed in gel filtration or centrifugation or SAXS or the like) could help flesh out these types of questions. Hope this helps, Kushol Kushol Gupta, Ph.D. Research Associate Van Duyne Laboratory - HHMI/Univ. of Pennsylvania School of Medicine kgu...@mail.med.upenn.edu 215-573-7260 / 267-259-0082 -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller Sent: Wednesday, May 25, 2011 10:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Histogram/Plot of Buried Surface Areas Dear Crystallographers, is anyone aware of a reference or plot addressing buried surface area (or PISA output values) versus veracity of a complex? I am trying to determine the physiological relevance of a crystallographically-observed assembly, and would love to put my PISA output in the context of verified complexes versus crystal contacts. Thanks, Jacob ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: j-kell...@northwestern.edu *******************************************
