Dear all, I know I could use your help to work more effectively so here comes my questions.
I am building a fairly long polypeptide chain (quite a few hundreds of aa) in Coot. I got the phases from Phenix, which traced much of the main chains for me already, with each fragments assigned with numbers that are apparently mismatched with the linear protein sequence. So, I begin to mutate/fit these Ala fragments (one piece at a time) into correct residues using Coot and also try to correct their numbers by doing "renumber residues". The outcome is that now I have overlapped numbers as I move along, prohibiting me to do real-space refinement effectively. How to get around with this? Thank you! Also, are there any easily accessible programs that could help me build these side-chains based on the very good experimental density map currently at 2.3A? Thanks, Jacob Wong
