Olá Carmela, see the recent paper from Müller-Dieckmann et al. about trimethylamine n-oxide in IUCr Journal of Applied Crystallography. I think it might as well be protein crystals, is it a big effort to have them tested down the "road" at the ESRF?
In my experience the methylated PEG makes a difference, but does not give "small molecule" crystals. Hope that helps a little, Harm --- Harm Otten Department of Chemistry Universitetsparken 5 2100 Copenhagen Denmark # +45 35 32 02 89 fax +45 35 32 03 22 email h...@chem.ku.dk Please consider the environment before printing this email. 2011/3/23 Carmela García <carmela.gar...@usc.es>: > Dear All, > > recently I got some crystals using the following condition: > 0.20 M trimethylamine n-oxide > 0.1 M Tris-HCl pH 8.5 > 20% (w/v) PEG MME 2000 > We have little experience with PEG MME and trimethylamine n-oxide, so I am > wondering how likely they are to be protein and would welcome hearing about > any other experiences people have with these precipitants/additives. The > protein was at around 40 mg/ml in 10 mM Tris-HCl pH 8.5, there may have been > a trace of nucleic acid present (i.e. A260/A280 around 0.6). > I don't have easy access to the crystals because they are in Grenoble at the > HTX service of EMBL. > > Greetings, > Carmela García Doval > Dpto de Estructura de Macromoleculas > Laboratorio M-4 > Centro Nacional de Biotecnologia > Campus Cantoblanco > c/Darwin 3 > E-28049 Madrid, Spain > carmela.gar...@cnb.csic.es > cgdo...@gmail.com >
