Dear All, recently I got some crystals using the following condition: 0.20 M trimethylamine n-oxide 0.1 M Tris-HCl pH 8.5 20% (w/v) PEG MME 2000 We have little experience with PEG MME and trimethylamine n-oxide, so I am wondering how likely they are to be protein and would welcome hearing about any other experiences people have with these precipitants/additives. The protein was at around 40 mg/ml in 10 mM Tris-HCl pH 8.5, there may have been a trace of nucleic acid present (i.e. A260/A280 around 0.6). I don't have easy access to the crystals because they are in Grenoble at the HTX service of EMBL.
Greetings, *Carmela García Doval Dpto de Estructura de Macromoleculas Laboratorio M-4 Centro Nacional de Biotecnologia Campus Cantoblanco c/Darwin 3 E-28049 Madrid, Spain carmela.gar...@cnb.csic.es cgdo...@gmail.com *
