Hi,
they buy it from some small company, idea being the applicability to
SLS, optimized for minimized bleeding/sheding or material
from the column, which will show up only in the light scattering
detector.
but i have to say some of our proteins stick to their columns
(happened during the demo), whereas they dontt to TOSOH,
on the other hand some proteins, as mentioned dont like silica at all
--- so you have to try, or if you know GE columns work for you
(i have some proteins that like to stick to sacharide based things...)
can go with them quite ok. Few tens of micrograms is
certainly enough on a 10/300 S200 for instance... narrow bore silica
columns (eg 4.8 mm TOSOH) appear to be bit limiting since
the flow rate would be better to be bit faster than what you can get
there (<0.4 ml/min vs 0.5-1ml/min on S200 superdex)
Tommi
On Mar 8, 2011, at 6:56 PM, Jacob Keller wrote:
So from where does Wyatt outsource their columns?
JPK
On Tue, Mar 8, 2011 at 10:49 AM, Sally Pham Thanh Van
<sally.pha...@gmail.com> wrote:
I should say clearer that I did a comparison between GE (Superdex
200
10/30) and the Wyatt column of the same separation range on AKTA
connected with MALS. The Wyatt column gave better resolution for size
exclusion chromatography as well as signal-noise ratio for light
scattering. So I think it is worth for you to try it. They offer
money-back guarantee.
Best wishes,
Sally.
On Tue, Mar 8, 2011 at 3:36 PM, Sally Pham Thanh Van
<sally.pha...@gmail.com> wrote:
Dear Sebastiano,
From my experiences, Wyatt columns are the best.
Best wishes,
Sally.
On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato
<sebastiano.pasqual...@gmail.com> wrote:
Dear all,
I was wondering if somebody could help me out by suggesting the
"best" column to be used in a Static Light Scattering (I guess it
would be the same for a Multi Angle Light Scattering) instrument.
We were suggested using a silica-based column, with very high
separation properties, but it seems that these columns are highly
sensitive to (even slightly) basic pH's. Even running the column
in PBS, it looks like injecting samples at pH 8.0 ruins the
column resin, making it unusable.
On the other hand, GE Healthcare columns would require a huge
amount of material to be loaded.
What are you guys using in your instruments?
Thanks a lot in advance for the feedback,
best,
ciao
Sebastiano
--
Sebastiano Pasqualato, PhD
Crystallography Unit
IFOM-IEO Campus
Cogentech - Consortium for Genomic Technologies
via Adamello, 16
20139 - Milano
Italy
tel +39 02 9437 5172
fax +39 02 9437 5990
--
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
Tommi Kajander, Ph.D.
Structural Biology and Biophysics
Institute of Biotechnology
University of Helsinki
Viikinkaari 1
(P.O. Box 65)
00014 Helsinki
Finland
p. +358-9-19158903
tommi.kajan...@helsinki.fi
