I should say clearer that I did a comparison between GE (Superdex 200 10/30) and the Wyatt column of the same separation range on AKTA connected with MALS. The Wyatt column gave better resolution for size exclusion chromatography as well as signal-noise ratio for light scattering. So I think it is worth for you to try it. They offer money-back guarantee.
Best wishes, Sally. On Tue, Mar 8, 2011 at 3:36 PM, Sally Pham Thanh Van <sally.pha...@gmail.com> wrote: > Dear Sebastiano, > > From my experiences, Wyatt columns are the best. > > Best wishes, > Sally. > > On Tue, Mar 8, 2011 at 2:03 PM, Sebastiano Pasqualato > <sebastiano.pasqual...@gmail.com> wrote: >> Dear all, >> I was wondering if somebody could help me out by suggesting the "best" >> column to be used in a Static Light Scattering (I guess it would be the same >> for a Multi Angle Light Scattering) instrument. >> We were suggested using a silica-based column, with very high separation >> properties, but it seems that these columns are highly sensitive to (even >> slightly) basic pH's. Even running the column in PBS, it looks like >> injecting samples at pH 8.0 ruins the column resin, making it unusable. >> On the other hand, GE Healthcare columns would require a huge amount of >> material to be loaded. >> What are you guys using in your instruments? >> Thanks a lot in advance for the feedback, >> best, >> ciao >> Sebastiano >> >> -- >> Sebastiano Pasqualato, PhD >> Crystallography Unit >> IFOM-IEO Campus >> Cogentech - Consortium for Genomic Technologies >> via Adamello, 16 >> 20139 - Milano >> Italy >> >> tel +39 02 9437 5172 >> fax +39 02 9437 5990 >> >
