Hi Hua, maybe crystallization by reducing ionic strength could work in this case. See for one prominent example the crystallization of Photosystem I by reducing the salt concentration of the mother liquor by slowly adding water (Fromme and Witt, Biochim Biophys Acta, 1365 (1998) 175-184). Greetings,
Jan ------- Dr. Jan Kern Lawrence Berkeley National Laboratory Physical Biosciences Division One Cyclotron Road, MS 66-326 Berkeley, CA 94720 On Sat, Feb 26, 2011 at 6:13 PM, Hua Yuan <foxso...@gmail.com> wrote: > Dear CCP4 community members, > > I've been trying to crystallize a protein complex that's very sensitive to > ionic strength, i.e., lower salt (~0.3M) will cause precipitation of the > complex but higher salt (~0.5 M) breaks the complex apart. The interaction > that holds the complex is probably mainly ionic type. > The crystals I got so far has only one component of the complex from which > all the crystallization conditions have high salt such as 2M Ammonium > Sulfate in them. Besides repeatly screening many crystallization > conditions, I was wondering whether is any way to work around this problem. > Your suggestions would be greatly appreciated! > > Thanks, > > Hua > > >
