Have you tried to crosslink your complex after purification and see if you can crystallize that ? Glutaraldehyde comes to mind but also DMA, DMS or DMP.
Jürgen On Feb 26, 2011, at 9:13 PM, Hua Yuan wrote: Dear CCP4 community members, I've been trying to crystallize a protein complex that's very sensitive to ionic strength, i.e., lower salt (~0.3M) will cause precipitation of the complex but higher salt (~0.5 M) breaks the complex apart. The interaction that holds the complex is probably mainly ionic type. The crystals I got so far has only one component of the complex from which all the crystallization conditions have high salt such as 2M Ammonium Sulfate in them. Besides repeatly screening many crystallization conditions, I was wondering whether is any way to work around this problem. Your suggestions would be greatly appreciated! Thanks, Hua ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
