Also try lower symmetry space groups. 36% solvent, while not unheard
of, is on a high end.
On Mon, 2011-02-07 at 02:49 -0800, Md. Munan Shaik wrote:
>
> Dear all,
> I have a question regarding the refinement and density map.
>
>
> My protein is 261 amino acids long and crystalize very nicely with
> very high resolution . There is no multiple spot in the image and
> diffraction looks amazing. I collected a dataset at 1.38 resolution
> and the space group is P43, overall Rmerg is 0.02 (most likely the
> space group is P4122, but then the solvent content is less than 16%
> for one molecule in the assymmetric unit, that are unlikely), so I
> processed the image in P4 (36% solvent) and run molecular replacement
> with a model that have 42 sequence identity. I got a solution in P43
> that are good enough in some part but in the map there are many gap at
> even lower sigma level. I tried to refine and Rfree stacked at 36
> along with Rwork, which is 33.
>
>
> I also checked the degradation patteren of the protein in the crystal
> and it looks not degraded and full length protein crystalize.
>
>
> Is there anybody can suggest me anything that I can try?
>
>
>
>
>
> Regards,
>
>
> Md. Munan Shaik
> PhD Student
> Department of Biotehnology
> School of Bioscience and Biotechnology
> via G. Colombo 03
> Padova 35131, Italy
> Mobile: 00393275671896
> E-mail: munanbt2...@yahoo.com
> munan.sh...@unipd.it
>
>
>
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
