If you are afraid by the possible low incorporation of selenium in your protein, and if you have some cysteines into your sequence, you can try the double labeling --> Structure, Vol. 11, 1359–1367, November, 2003.
It works at the first try for me and I had no change in crystallization conditions. Michel. 2011/1/6 Yibin Lin <yyb...@gmail.com> > Dear all, > I try to express selenomethinine-labeled protein in E.coli methinoine > auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18 > hours at 37 degree. I don't why the colour of medium vary from colorless to > yellow. Could anybody tell me it is normal or not? > > Thanks a lot! > > Lin >
