I think you should look at pubmed id 14604526, it works well for me. You should obtain the cells if you contact the authors.
Michel. Le 18 oct. 2010 à 17:27, Daniel Bonsor <bon...@bbri.org> a écrit : > Have you looked at the Keio collection. Though they are not compatible with > T7 promoters, you can use the λDE3 Lysogenization Kit to add the T7 > polymerase. > > A possible source is from Yale http://cgsc.biology.yale.edu/Auxotrophs.php > though of course there are others. > > > Dan
