Dear Sebastiaan, isn't it the editor rather than the referees whom you have to convince? And if the editor does not even understand how SDS-PAGE works and still considers this a reason not to publish your article against your own expertise, maybe it is worth changing the journal.
Finally, since referees know the names of the authors of the articles they are refereeing, you may need a lot more than a couple of references to appease them after you called them "bloody-minded" in a public email forum. My two cents, Tim On Thu, Oct 28, 2010 at 01:16:59PM +0200, Sebastiaan Werten wrote: > Dear all, > > we have a his-tagged protein that shows a minor accompanying band in > SDS-PAGE, just above the main band. According to all other methods > available to us the material is homogeneous, the protein has the correct > mass in MALDI-TOF, epitopes are recognized, etc. etc. > > I know that the additional band is a very common artifact with > his-tagged proteins, but I was wondering if anyone is aware of a paper > that formally describes the phenomenon, as we need to appease a couple > of rather bloody-minded referees. > > Thanks very much for any suggestions, Seb. > > -- > Dr. Sebastiaan Werten > Institut für Biochemie > Universität Greifswald > Felix-Hausdorff-Str. 4 > D-17489 Greifswald > Germany > Tel: +49 38 34 86 44 61 > E-mail: sebastiaan.wer...@uni-greifswald.de -- -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen phone: +49 (0)551 39 22149 GPG Key ID = A46BEE1A
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