Remember you need to look at half the contour height in a mFo map
compared to a 2mFo-DFc map - the same domain should show up but at a
lower relative contour level in both maps.
REFMAC calculates a similar WCNG and you can look at the graph of m
after refinement to see how close it is to 1. If the structure is well
refined except for a missing domain I suspect it will be ~ 1
Eleanor
Hailiang Zhang wrote:
Actually I cut a small domain from the well-defined structure (just for a
test). The missing part showed in 2mFo-DFc map but not in both mFo and Fo
maps, and the mFo and Fo maps are so close so that I wonder whether figure
of merit generated by SIGMAA helps or not in this situation...
Best Regards, Hailiang
On Tue, 2010-08-31 at 13:15 -0400, Hailiang Zhang wrote:
Is the difference
between mFo and Fo maps supposed to be very small?
For an essentially correct model, yes. The major advantage of (2mFo-DFc)
maps is suppression of model bias, so if you don't see much difference
then your model is very well refined. For illustration, introduce a
systematic error on purpose and see which map gives you better result.
--
"I'd jump in myself, if I weren't so good at whistling."
Julian, King of Lemurs
