What concentration/ratio of your inhibitor did you use ? Also check this out: (short soaks, see time course experiment) Bosch et al. Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase. J Med Chem (2006) vol. 49 (20) pp. 5939-46
Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Aug 28, 2010, at 1:40 AM, 胡小鹏 wrote: > Dear all, > We are working on protein-inhibitor complex crystal structures, although > we did get nice crystals and soaked them with inhibitor for several days, all > structures we obtained were empty!!There are nothing at the active site. For > several crystals. their color turned very clearly from colorless to yellow > the color of the inhibitors, nothing found either. > These inhibitors work very well in enzyme assay. We, especially our > students, are very depressed... > Any suggestions will be really appreciated! > > > > > Xiaopeng Hu, D.Sc., > School of Pharmaceutical Sciences > Sun Yat-sen University, > Higher Education Mega Center > Guangzhou, Guangdong, China 510006 > Tel. +86 020 39943032
