Hello, If the data are being collected at cryogenic temperatures and no inhibitor has been included in the cryoprotectant, perhaps the inhibitor would diffuse out of the active site?
Ping +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ T z u - P i n g K o < kotp...@gate.sinica.edu.tw > Institute of Biological Chemistry Academia Sinica, Nankang, Taipei 11529, Taiwan Tel. +8862 2785 5696 ext 5011 FAX. +8862 2788 9759 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ ---- 原始郵件 ---- >Date: Sat, 28 Aug 2010 13:40:11 +0800 >From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> (on behalf of 胡小. ><huxp...@mail.sysu.edu.cn>) >Subject: [ccp4bb] Problem with getting protein-inhibitor complex structure >To: CCP4BB@JISCMAIL.AC.UK > >Dear all, > We are working on protein-inhibitor complex crystal structures, although > we did get nice crystals and soaked them with inhibitor for several days, all > structures we obtained were empty!!There are nothing at the active site. For > several crystals. their color turned very clearly from colorless to yellow > the color of the inhibitors, nothing found either. > These inhibitors work very well in enzyme assay. We, especially our > students, are very depressed... > Any suggestions will be really appreciated! > > > > >Xiaopeng Hu, D.Sc., >School of Pharmaceutical Sciences >Sun Yat-sen University, >Higher Education Mega Center >Guangzhou, Guangdong, China 510006 >Tel. +86 020 39943032
