Looks atypical for 3-me-Lys, M3L, (usually in natively methylated products) http://www.ruppweb.org/garland/gallery/Ch2/pages/Biomolecular_Crystallograph y_Fig_2-27.htm
nor fits dimethyllysine, MYL, (via chem. red) http://www.ruppweb.org/garland/gallery/Ch4/pages/Biomolecular_Crystallograph y_Fig_4-19.htm BR PS: ...and dont forget to remove those waters later ;-) -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of J. Preben Morth Sent: Tuesday, June 29, 2010 1:44 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] density quiz Hi Christian One guess would be one of the possible post translational modifications seen for Lysine. acetylation or a triple methylation. However propionylations and butyrylations have also been observed on lysine. It looks like you could build in waters, you may want to leave this lysine till later and see if more clear density show up. good luck Preben On 29/06/2010, at 21.52, Christian Biertuempfel wrote: > > > Dear all, > Does anyone have an idea what this density at a lysine residue could > be? > The structure is a protein-DNA complex and I suspect that something > left-over from the DNA synthesis reacted with the amino group even > though the DNA substrate was purified. > > christian > > p.s. The protein has seen the following substances during purification > and crystallization: Leupeptin, Pepstatin, AEBSF, MES, Tris, HEPES, > DTT, > EDTA, imidazole, glycerol, PEG 2K-MME, Mg2+, Na+, K+, Ca2+, Cl-. > > > _______________________________________________________________________ > > Dr. Christian Biertümpfel > Laboratory of Molecular Biology > > NIDDK/National Institutes of Health phone: +1 301 402 > 4647 > 9000 Rockville Pike, Bldg. 5, Rm. B1-03 fax: +1 301 496 > 0201 > Bethesda, MD 20892-0580 > USA > _______________________________________________________________________ > < > density_quiz1 > .png > > > < > density_quiz2 > .png><density_quiz3.png><density_quiz4.png><density_quiz5.png> Jens Preben Morth, Ph.D Aarhus University Department of Molecular Biology Gustav Wieds Vej 10 C DK - 8000 Aarhus C Tel. +45 8942 5257, Fax. +45 8612 3178 j...@mb.au.dk website: http://person.au.dk/en/j...@mb.au.dk
