Hi Murugan, One useful indicator of raw anomalous signal is the ANOMPLOT graph from Scala - this shows the differences between reflections compared with the expected differences. If the gradient of the plot is 1 there's no more differences that you would expect. If the gradient is more than one there is (or may be.) - also check out the merging statistics as a function of batch, if there's significant radiation damage this may mess things up.
Scala writes out the gradient (assuming you told it anomalous on) in the summary Another rule-of-thumb is the resolution limit where cc-anom is > 0.5. The most practical indicator of the anomalous signal is of course the success or failure of the subsequent phasing :o) Best wishes, Graeme On 29 June 2010 10:05, Vandu Murugan <wandumuru...@gmail.com> wrote: > Dear all, > I have collected a 2.7 angstrom home source data with Cu-Kalpha source > for a protein with 6 cysteines, with a multiplicity of around 23. I need to > know, is there any significant anamolous signal present in the data set, > since there is no good model for my protein. Can any one tell, which > program to run, and what parameter to see? Thanks in advance. > > cheers, > Murugan >
