Vandu Murugan wrote:
Dear all,
I have collected a 2.7 angstrom home source data with Cu-Kalpha
source for a protein with 6 cysteines, with a multiplicity of around
23. I need to know, is there any significant anamolous signal present
in the data set, since there is no good model for my protein. Can any
one tell, which program to run, and what parameter to see? Thanks in
advance.
cheers,
Murugan
When processing the data, ensure that Bijvoet pairs are not merged. The
data processing software should provide you with an R-ano value and that
is already a start. The values provided should tell you if you have an
anomalous signal or not. You may also have to play with the number of
frames to integrate in order to obtain an "optimal" anomalous signal in
the resulting data set.
There are several publications describing Sulphur SAD, but one of them
which is freely available on the Web can be found here:
http://www.stoe.com/pages/brochure/labnote_genix_cu.pdf , Schiltz M (pp
4-6).
Good luck!
Fred.
