Hi Andre,
We saw this type of crystals quite often during crystallization of our 300 kDa
complex. To our experience these crystals diffract very poorly (~7-10 A) and
anisotropically.
They may have highly favorable crystal contacts in one direction than the other
two directions and grow very fast in favorable direction. To our experience we
could not improve the diffraction significantly for these crystals.
However, it is possible that in case of small protein, the diffraction can be
improved.
Best luck,
ravi
Ravindra D. Makde
present address:
Scientific Officer E
High Pressure & Synchrotron Radiation Physics Division
Bhabha Atomic Research Centre,
Trombay, Mumbai, India.
Tel: +91-22-25506754 (Res.),
"All that we are is the result of what we have thought.
The mind is everything. What we think, we become"
Buddha
--- On Wed, 6/2/10, Andre Ambrosio <andre.ambro...@cebime.org.br> wrote:
From: Andre Ambrosio <andre.ambro...@cebime.org.br>
Subject: [ccp4bb] Off-topic: hollow protein crystals
To: CCP4BB@JISCMAIL.AC.UK
Date: Wednesday, June 2, 2010, 3:07 PM
Dear all,
We have recently obtained crystals from a small protein, and
interestingly, at least for me, they are hollow trigonal rods (please see
pictures attached).
Just out of curiosity, has anybody ever seen such feature
for protein crystals before?
Regards,
-Andre.
