4. protein monitoring
Dear Yogesha,You can use the absorbance at 215 or 220 nm to follow your peptide during purification. Compounds like DTT and EDTA can increase the noise at those wavelengths, go to 220 nm if you have things like that in your purification buffers.
Quantification of the peptide will have to be performed by Bradford or other colorimetric method, and I guess you can use that quantification to find out the extinction coefficient of your peptide at 215-220 nm.
Luck, Carlos
