Hi all I am trying to do molecular replacement with low resolution (4Å) using Molrep and Phaser.
Overall R-factor is 11.3%, completeness 95.4%, I/sigma 2, and Chi^2 0.95. Identities between my protein and templates were more than 80%. I couldn’t get correct solution. Rotation function, translation score, and contrast were low, and they had no significance, though I changed the range of resolution. Molrep suggested solution coordinates clashed with symmetry molecules. I tried MR after remove clashed regions, but another clashes happened. In the case of phaser, there were many clashes, too. Please, give me any suggestion. Should I concern about any options when I run MR programs? Hope you guys will be interested to answer!!!!!! Thanks in advance -- INTEKHAB ALAM LABORATORY OF STRUCTURAL BIOINFORMATICS KOREA UNIVERSITY, SEOUL
