There are a couple of additional factors not taken into account here. 1. LN2 versus frozen in strem or propane etc 2. did you try to flash anneal the larger crystal 3. smeary diffraction from the big crystal or not ? 4. how much residual solvent was around your crystal when freezing ?
In general smaller crystals are anyhow better in my hands. Jürgen On Apr 14, 2010, at 5:36 PM, syed ibrahim wrote: > Hi All > > I had two crystals grown in same well, one is small and other is 10 times > bigger. I treated both crystal in same cryo and same time. The smaller one > diffracted to 2.5A and the bigger one to 6-7A. I was expecting the bigger one > to diffract high resolution. > > I assume the bigger crystal might have lot of solvent which prevent for high > resolution. If it is true what could be the best way to dehydrate crystal > without affecting crystal quality? > > Thank you > > Syed > > PS: Taken care of less solvent to be present in the loop > > > - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/
