Dear Tim,
Although it is not routine, it can be done in mammalian cells too! For example,
have a look at this:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf
HTH,
Luca
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Luca Jovine, Ph.D.
Karolinska Institutet
Department of Biosciences and Nutrition & Center for Biosciences
Hälsovägen 7, SE-141 57 Huddinge, Sweden
Voice: +46.(0)8.6083-301 FAX: +46.(0)8.6081-501
E-mail: luca.jov...@ki.se
W3: http://jovinelab.org
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On Mar 23, 2010, at 1:25 PM, Tim Gruene wrote:
> Dear all,
>
> since I am currenty preparing a lecture on crystallography I am wondering
> about
> the status quo of the production of SeMet proteins.
> In 2003, if I remember correctly, it was possible to express SeMet proteins in
> E.coli and insect cells.
>
> Has this been extended to other systems, and if so, which ones?
>
> Thanks a lot,
> Tim
>
> --
> --
> Tim Gruene
> Institut fuer anorganische Chemie
> Tammannstr. 4
> D-37077 Goettingen
>
> GPG Key ID = A46BEE1A
>
