Hello Dave, 

I have good experience with concentrations between 10% and 15%
2,3-butanediol. My typical starting concentration is 12.5%.

Good luck.

Eike 


Am 23.03.10 08:27 schrieb "David Briggs" unter <drdavidcbri...@gmail.com>:

> 2,3-BD users, 
> 
> What sort of concentrations are you using?
> 
> Dave
> 
> --
> Delivered via an Android.
> 
>> On Mar 22, 2010 11:45 PM, "Steve Tuske" <tu...@cabm.rutgers.edu> wrote:
>> 
>> Hi Ulrike,
>> 
>> 2,3-butanediol has worked very nicely as a cryoprotectant.  In my hands it
>> mostly seems to decrease mosaicity.  I was able to switch from a
>> three-step soaking protocol with glycerol and glucose to a quick (30
>> second to one minute) soak with 2,3-BD.
>> 
>> It's a winner.
>> 
>> Steve
>> 
>> I
>> 
>>> > Hello Ulrike, > > in our lab 2,3-Butanediol has had a good reputation. > >
>>> Tim > > On Mon, Mar 22...
> 

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