Hi Everyone,
I hope everyone gets this email.
Below are the two answers I got on how to solve my problem using ccp4.I
actually emailed another person who wanted to know how I did it. So I got
to transfer what I learned immediately. But his email was offline not
through the forum.
The answers to my queries on how to make an anomalous difference fourier
map using CCP4.
The first answer is the one that I used, but I am attaching all the answers
that I got.
1)
Hi Ivan,
coot can do that quite nicely.
Make sure you have the DANOs in the same file as the phases, else use CAD to
merge files.
Then 'load mtz', go to 'Expert mode', select DANO as amplitudes, PHWT as
phases, unselect 'difference map' and contour at somewhere between 3-5
sigma.
2)
Xaravich,
Just to clarify you are using:
PHI=PHIC (SFALL), DANO=DANO (data), Sigma=SIGDANO (data) and WEIGHT=FOM
(SFALL)..to my recollection, you shouldn't be using FCalc..
I've never used SFALL to generate phases. I would use the PHIC from the
output mtz of refmac..but I think this is the same thing.
All the other steps look correct to me.
thanks,
Ivan
