You would have to cheat a little bit, and relabel the second domain chain id to something different. Or you can split the pdb file into two. That would still be necessary for using the EBI PISA server at
http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html as I have just been doing. In my case I wanted the interface between multi- chain domains, so I had to pretend they were one chain each. It works alright. Pierre ******************************************************** Dr. Pierre Rizkallah, Senior Lecturer in Structural Biology, WHRI, School of Medicine, Cardiff University, Heath Park, CF14 4XN email: rizkall...@cf.ac.uk phone + 44 29 2074 2248 >>> Jane Bailey <jbailey.x...@googlemail.com> 19/02/10 3:44 PM >>> Dear all, I am trying to calculate the domain-domain contact surface from one chain. I see AREAIMOL to only tell you the accessible surface area/residue. Could any other software/webserve could specify residues in the contact surface and calculate the total contact area? Thanks J.
