Dear all,
I am working on a structure with resolution up to 2.7 Ang.in space group P43212. I am able to fit two molecules of this 10 Kd protein in the asymmetric unit using PHASER, however the solvent content was only 30 % and matthews coefficient was around 1.70. After molecular replacement the value of R/Rfree factors was 40.00/48.00 and electron density Model was fitting well. As the refinement and mutations were introduced the R and R free factor remain stuck at 35 and 48 even after many cycles. Thanx in advance for your valuable suggestions. Regards Mirage singh DONT EXPLAIN !!,FRIENDS DONT NEED IT ,ENEMIES WONT BELIEVE IT
