I need to dephosphorilate blunt ends and, surfing on internet I found this two enzymes: - Shrimp Alkaline Phosphatase (SAP) - Calf Intestinal Alkaline Phosphatase (CIP)
Have you any experience about these phosphatases, I mean wich is the best for blunt ends? All the best Nenci Simone ------------------------------------------------- Dept. Genetics and Microbiology University of Pavia Via Ferrata 1, 27100 Pavia Italy Tel. +39-0382-985535/985534 Fax +39-0382-528496 Web www.unipv.it/biocry
