Hi,
I have 20 identical monomers in my asu of a 2.5A structure. We have previously model built all twenty monomers and used strict NCS in the refinement, however I would like to compare this with the maps generated by building one monomer into an averaged map and then replicating it nineteen times for the refinement. On a previous structure with just two monomers I did this to good effect using coot to make my average map and then pymol to replicate the second monomer, however with twenty monomers things are a bit more complicated. So: 1. Can the map averaging function in coot cope with averaging across twenty monomers or is there another better program to use? 2. What program can I use to generate the nineteen NCS symmetry operators and then apply them to the monomer I am building? I'm guessing I'll need a script to do this - is there a webpage/tutorial that explains how to do what I imagine must be a fairly common procedure? Thanks, Simon
