It should be "In situ" and not 'In site'. Sorry for the typo error. -Partha
On Sun, Oct 11, 2009 at 9:59 PM, Parthasarathy Sampathkumar < spart...@gmail.com> wrote: > Hi Jing, > > Methods to perform In site proteolysis are available in the following > publications: > > http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0005094 > > > http://www.nature.com/nmeth/journal/v4/n12/abs/nmeth1118.html > > Good Luck, > -Partha > > > > On Sun, Oct 11, 2009 at 5:13 PM, jwangliang <jwangli...@gmail.com> wrote: > >> Hello, >> >> I am sorry to put this question which didn’t related to the CCP4 software. >> >> I couldn’t get the crystals from the protein only. So I want to try the >> different way to work it out. I hear that it is possible to set a crystal >> tray of protein with Trypsin protease. I will be very appreciated about who >> will provide some detail of this method about how to do this., e.g. How >> much trypsin shall I add into the protein, How long shall I mix protease >> with the proteins before I set the tray. Thanks for all. >> I will also put on the summary of all the answers. >> >> Another question is about protein express in insect cell. 10% of my >> protein was degradated after 1st purification. Is anyway that I can >> prohibit this degradation? >> >> >> >> Thanks >> >> >> Jing >> > >
