Hi Jing, Methods to perform In site proteolysis are available in the following publications:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0005094 http://www.nature.com/nmeth/journal/v4/n12/abs/nmeth1118.html Good Luck, -Partha On Sun, Oct 11, 2009 at 5:13 PM, jwangliang <jwangli...@gmail.com> wrote: > Hello, > > I am sorry to put this question which didn’t related to the CCP4 software. > I couldn’t get the crystals from the protein only. So I want to try the > different way to work it out. I hear that it is possible to set a crystal > tray of protein with Trypsin protease. I will be very appreciated about who > will provide some detail of this method about how to do this., e.g. How > much trypsin shall I add into the protein, How long shall I mix protease > with the proteins before I set the tray. Thanks for all. > I will also put on the summary of all the answers. > > Another question is about protein express in insect cell. 10% of my > protein was degradated after 1st purification. Is anyway that I can > prohibit this degradation? > > > > Thanks > > > Jing >
