Hi Camille, I don't know if you have any protein labs around you but if someone is using rosetta or codon-plus type expression Ecoli strains those cells usually contain a plysS plasmid derivative that is chloramphenicol resistant and carries the gene encoding lysozyme among other things (plus the rare tRNA genes). If they don't have the plasmid at hand you can still grow the cells and make a miniprep of the plasmid (low copy) to have a lysozyme gene in your hands.
Hope this helps. Let me know if you can't find it. -- Pascal F. Egea, PhD Assistant Professor UCLA, David Geffen School of Medicine Department of Biological Chemistry 314 Biomedical Sciences Research Building office (310)-825-1013 lab (310)-825-8722 email pe...@mednet.ucla.edu
