Dear all, I run refmac5 on TLS & restrained refinement mode. my pdb input file contain anisotropic temp for the amino acid and isotropic temp for the cofactors and water. The refinement parameters is set on mixed isotropic/ anisotropic temp and in TLS parameters the check box add TLS contribution is marked. In the pdb output the cofactor stay with isotropic temp BUT the water get anisotropic temp. How can i leave the water with the isotropic tepm (i work on win version)
thanks Elad Elad Binshtein Ph.D student Department of Life Science Ben Gurion University of the Negev Ph: 972-8-6461325
