Hi, I've used the CFX96 with the FRET channel for DSF and found it to work well.
Gary -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of ar...@xtals.org Sent: Monday, June 01, 2009 11:47 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] BioRad CFX96 for thermofluor/DSF? Use the FRET channel - it's ideal for SYPRO orange. Artem > Sorry Charlie for the late reply. > I'm using the CFX96 5 channel with Sypro and other dyes. I tested the > machine against the Eppendorf product nd decided to go for the Biorad > version as running identical samples gave better results on the > Biorad. This could be a machine specific problem but on a 96 well > plate the detection level was ~1.5x better, you can use the HEX > channel for detection. > > Jürgen > > On 29 Apr 2009, at 03:06, Charlie Bond wrote: > >> Hi Folks, >> >> Has anyone here had any success running a thermofluor (differential >> scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange >> as >> the fluorophore? >> >> Our local 'technical expert' tells us it should work and gave us some >> settings to test, but was very sketchy about any detail, and in a test >> we ran we got some strange results (every well that contains SYPRO >> orange - even those with no protein - have a peak in fluorescence at >> 58oC). >> >> Before we tinker, or go and persuade a friendly molbiol lab to let us >> play with the filters in their iq5, I thought I'd see if anyone here >> had >> any advice. >> >> I'll summarise any responses I get. >> >> Cheers, >> Charlie >> >> -- >> Charlie Bond >> Professorial Fellow >> University of Western Australia >> School of Biomedical, Biomolecular and Chemical Sciences >> M310 >> 35 Stirling Highway >> Crawley WA 6009 >> Australia >> charles.b...@uwa.edu.au >> +61 8 6488 4406 > > - > Jürgen Bosch > Johns Hopkins Bloomberg School of Public Health > Biochemistry and Molecular Biology, W8708 > 615 North Wolfe Street > Baltimore, MD 21205 > Phone: +1-410-614-4742 > Lab: +1-410-614-4894 > Fax: +1-410-955-3655 > http://web.me.com/bosch_lab/ >
