Hi Folks,
Has anyone here had any success running a thermofluor (differential
scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange as
the fluorophore?
Our local 'technical expert' tells us it should work and gave us some
settings to test, but was very sketchy about any detail, and in a test
we ran we got some strange results (every well that contains SYPRO
orange - even those with no protein - have a peak in fluorescence at 58oC).
Before we tinker, or go and persuade a friendly molbiol lab to let us
play with the filters in their iq5, I thought I'd see if anyone here had
any advice.
I'll summarise any responses I get.
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
charles.b...@uwa.edu.au
+61 8 6488 4406
