Hi Matthew,
Two anecdotes:
For one protein we had protein in pH7 with 10 mM Mn2+ and just set up
crystal trials as normal. Although at higher pH excess Mn precipitated
as MnO2, we still had Mn (2+, presumably) in the active.
For another protein, we grew apo crystals at pH8 and then gradually
reduced the pH (transferred crystals to new drops) and they survived to
the point of pH7 where we supplemented with Mn2+.
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
charles.b...@uwa.edu.au
+61 8 6488 4406
