David Garboczi wrote:
Have large proteins (300+ kD) been successfully produced in E. coli? Which ones?
have a look at Suo et al., 2000, PNAS, 14188-14193; (350 kDa); or Pan et al., 1999, NAR, 1094-1103; ('only' 190 kDa). klaus
Dave
-- ************************************************************************ Dr. Klaus Scheffzek European Molecular Biology Laboratory (EMBL) Structural and Computational Biology Unit Meyerhofstr. 1 69117 Heidelberg, Germany Phone: (49) 6221 387 401 Fax: (49) 6221 387 306/519 E-mail: scheff...@embl.de Web: www.embl.de ************************************************************************