On Monday 02 February 2009, Vesna Serrano wrote: > Dear all, > I have a problem with a SAD data set. My crystal is very sensitive to > radiation damage, so I have collected 2 110 frame data sets, 180 deg > apart. I have processed the data with HKL2000 and it turns out that the 2 > sets have very different scales. My question is whether it would be > possible to normalize the scales for these two data sets, so that I can > use anomalous information in order to localize the anomalous scatterer in > my structure.
You would do better to go back to the unscaled data, and feed the unmerged intensities from both datasets into the SAD phasing program. Let it do the scaling jointly. Better yet would have been to collect the data in smaller wedges using "inverse beam mode". -- Ethan A Merritt Biomolecular Structure Center University of Washington, Seattle 98195-7742
