You don't have EDTA left over from your protease inhibitor, do you?
Some commercial cocktails include it. You may have to read the fine
print.
James
On Jan 27, 2009, at 1:00 PM, Fred wrote:
Hi ccp4 list,
I am trying to purify a his-tag protein by metal affinity
chromatography. The protein was expressed in inclusion bodies and
its his-tag doesn't bind the Qiagen Ni resin in denatured conditions
(urea 8M or GndHCl 6M). Playing with NaCl and detergents didn't help
much.
Any help is appreciated.
Fred
